EZ 96 Plant DNA Protocol-Vacuum Manifold Processing-åŽå¼ºç”µå网
Note: The following protocol is based on using OBI's vacuum manifold (Product No. VAC-03).
1. Set up vacuum manifold by the following manufacturer's instructions.
2. Place waste collection tray inside the vacuum manifold, then place the EZ 96 ®
DNA plate on top part of the manifold.3. Apply the entire sample (including any precipitate that may have formed to an EZ 96 ®
DNA plate.Note: It is always good idea to mark the EZ 96 ®
DNA plate and collection plate at this stage so that they can be easily identified throughout the protocol.* Do not touch the rim of the wells with pipet tips to avoid crosscontamination.
4. Turn on the vacuum manifold and filter through the sample mixture by vacuum. Turn off the vacuum.
5. Add 700 ul DNA wash buffer into each well of the EZ 96 ®
(Dilute the DNA wash buffer with ETOH before use.)6. Wash the plate with another 700 ul DNA wash buffer by repeating step 5.
7. Repeat Step 6 by washing the plate with 400 ul 100% ethanol. Continue vacuum until the EZ 96 ®
DNA plate is completely dried.8. Remove the EZ 96 ®
DNA plate from manifold and tap hard on a stack of paper towels to remove any residue ethanol. Discard the flowthrough and collection plate.Note: It is very important to completely dry the EZ 96 ®
DNA plate before elution. If a swing bucket centrifuge and 96-well plate adaptor are available, centrifuge at 4,000 xg for 10 minutes to dry the plate.9. Assemble the manifold by placing a new 300 ul collection plate (supplied) inside the vacuum manifold. If an Omega VAC-03 is used, one 800 ul plate should be place under the 300 ul plate to give proper height for elution.
10 Place the EZ 96 ®
DNA plate atop the vacuum manifold.11. To elute the DNA, add 100 ul of preheated (65°C) Elution Buffer to each well using a multichannel pipet. Incubate for 5 min at room temperature. Apply the vacuum to elute the DNA into collection plate.
TIP: 100 ul water or TE buffer is sufficient to elute up to 85% of the DNA from each well of the EZ 96 ®
DNA plate. A second elution step with same 100 ul elutate containing DNA, reheated to 65°C, will increase yield by up to 10-15%.Total DNA yields vary depending on type and amount of sample. Typically, 5-10ug DNA with a A260/A280 ratio of 1.7-1.9 can be isolated using 10 mg dried tissue.
Medical Cable Assembly refers to the customized wiring harness applied to various medical equipment. The quality of medical cable assembly can be said to be the highest in the industry, because modern medical safety factor requirements are gradually increasing, and every component involved in medical equipment must undergo strict testing processes. In order to ensure the quality of medical cable assembly. To meet the standards, strict attention must be paid to the production and processing stage of medical Wire Harness .
1. Opening process of medical cable assembly: The accuracy of the opening process is directly related to the entire production schedule. Once errors occur, especially if the opening size is too short, it will lead to rework of all stations, which will take time and effort and affect production efficiency.
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We welcome you to contact our sales or engineering team to discuss the special needs of your application.
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Kable-X Technology (Suzhou) Co., Ltd , https://www.kable-x-tech.com